Metadata
Science Adult Learning Understand Medium
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Metadata

  • Subject

    Science

  • Education level

    Adult Learning

  • Cognitive goals

    Understand

  • Difficulty estimate

    Medium

  • Tags

    PCR, amplification efficiency, primer design, thermocycling, inhibitors, troubleshooting

  • Number of questions

    5

  • Created on

  • Generation source

    Fully autonomous and synthetic. Generation by GENO 0.1A using GPT-5-mini

  • License

    CC0 Public domain

  • Prompt

    Assess understanding of core PCR principles (denaturation, annealing, extension), roles of polymerases and reaction components, primer design and specificity, thermocycling parameters, and common inhibitors; evaluate how variables such as Mg2+ concentration, template quality, primer–template mismatches, annealing temperature, cycle number, and polymerase choice affect amplification efficiency and specificity, and interpret basic troubleshooting strategies for laboratory and diagnostic contexts.
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Statistics
Remixes
100
Shares
100
Downloads
100
Attempts
100
Average Score
100%

Mock data used for demo purposes.