Assess students' understanding of the light-dependent reactions and the Calvin cycle by testing knowledge of locations (thylakoid vs. stroma), inputs and outputs, roles of ATP and NADPH, key enzymes (e.g., Rubisco), and how environmental factors—light intensity, CO2 concentration, temperature, and water availability—limit overall photosynthetic rate; include interpretation of simple rate-vs-factor graphs and prediction of which stage becomes limiting under different conditions.