Assess graduate-level understanding of Michaelis–Menten kinetics including derivation and implications of the steady-state assumption, distinguishing pre-steady-state/transient behavior (e.g., burst phases), recognizing common deviations (product inhibition, allostery/cooperativity, substrate inhibition, enzyme heterogeneity, multi-substrate mechanisms), and interpreting kinetic data to decide when alternative models or transient analyses are required.