Assess graduate-level understanding of Michaelis–Menten kinetics including derivation from a mechanistic enzyme–substrate scheme using the steady‑state assumption, definitions and interpretation of Vmax, Km and kcat, limits of validity, and ability to recognize and distinguish common deviations (allosteric regulation, substrate inhibition, cooperativity) from experimental plots and parameter analyses (e.g., Lineweaver–Burk, Eadie–Hofstee, Hill).