Assess students' ability to apply Michaelis-Menten kinetics to calculate initial reaction rates and determine Vmax and Km from provided data (including Lineweaver-Burk or Eadie-Hofstee plots), identify and distinguish competitive, noncompetitive, uncompetitive, and mixed inhibition by their effects on kinetic parameters and plots, and interpret how these inhibitory mechanisms alter metabolic pathway flux and physiological outcomes. Include numerical calculations, graph analysis, and brief written explanations.