Assess students' understanding of molecular mechanisms of enzyme inhibition (competitive, noncompetitive, uncompetitive, irreversible, allosteric), their kinetic signatures (effects on Km and Vmax, Lineweaver–Burk interpretations), and the downstream consequences for metabolic pathway flux and regulation (feedback inhibition, substrate/product accumulation, and therapeutic inhibition). Items may require interpretation of kinetic data, prediction of pathway responses, and explanation of physiological or pharmacological examples at the undergraduate level.