Assess students’ ability to analyze enzyme assay data using Michaelis–Menten kinetics to: identify inhibitor type (competitive, noncompetitive, uncompetitive, or mixed); estimate Km and Vmax from plots or nonlinear regression; calculate Ki when applicable; and justify conclusions based on changes in kinetic parameters and graphical intersections using a provided dataset of initial velocities with and without inhibitor. Include numerical fits, relevant plots (e.g., Lineweaver–Burk or Eadie–Hofstee) and brief discussion of common pitfalls (reciprocal-plot distortion, substrate range).