Assess students' ability to analyze multi‑substrate enzyme mechanisms and allosteric regulation by interpreting steady‑state and pre‑steady‑state (transient) data to distinguish sequential vs ping‑pong mechanisms, estimate kinetic parameters (kcat, Km, Ki, kobs), recognize cooperativity and applicable allosteric models (Hill, MWC/KNF), design or critique stopped‑flow and steady‑state experiments, and draw mechanistic conclusions about rate‑limiting steps from provided datasets.