Assess graduate students' ability to analyze enzyme allosteric regulation and cooperativity from kinetic datasets: construct and interpret dose–response curves and Hill plots, calculate EC50 and Hill coefficients, distinguish positive/negative/noncooperative behaviors, fit and compare mechanistic (MWC, KNF) and empirical (Hill) models, evaluate goodness-of-fit and model selection criteria (AIC/BIC), recognize experimental artifacts and parameter identifiability limits, and propose model-driven experimental follow-ups.