Assess graduate-level understanding of molecular and kinetic mechanisms of enzyme allostery and cooperativity in metabolic regulation, including structural bases of conformational change and subunit interactions, classical models (MWC and KNF), quantitative descriptors (Hill coefficient, cooperativity constants), effects of activators/inhibitors on pathway flux, and interpretation of kinetic and binding data (sigmoidal vs hyperbolic curves) through conceptual questions and data analysis.