Assess students' understanding of mechanisms and physiological roles of allosteric regulation in metabolic enzymes, including molecular basis (allosteric sites, conformational change), kinetic effects (cooperativity, sigmoidal behavior, Hill coefficient), classic models (MWC/KNF), types of effectors (homotropic vs heterotropic, feedback inhibition), representative examples (PFK‑1, ATCase, glycogen phosphorylase), experimental approaches, and implications for metabolic homeostasis, disease, and therapeutic targeting.