[Test graduate-level ability to analyze initial-velocity enzyme assay data using Michaelis–Menten kinetics to identify inhibitor type (competitive, noncompetitive, uncompetitive, or mixed) and to calculate Ki. Students should interpret Lineweaver–Burk, Eadie–Hofstee, or nonlinear fits to extract Km and Vmax with and without inhibitor, determine the mechanism from changes in Km and Vmax, and compute Ki using appropriate equations or secondary plots given multiple inhibitor concentrations and reversible inhibition assumptions.]