Assess students' ability to apply Michaelis–Menten kinetics to velocity vs substrate concentration data (with and without inhibitor) to identify the inhibitor mechanism (competitive, noncompetitive/mixed, or uncompetitive) using appropriate analyses (e.g., Lineweaver–Burk or nonlinear fitting), calculate Km and Vmax changes, derive Ki from primary or secondary plots or algebraic relations, and justify conclusions with clear calculations and interpretation of enzyme efficiency.