Assess students’ ability to apply the Michaelis–Menten equation to determine Km and Vmax and to calculate inhibitor Ki from initial-rate enzyme kinetics data. Provide datasets of initial velocity versus substrate concentration with and without a known inhibitor concentration; require determination of Km and Vmax using appropriate methods (nonlinear regression or linear transforms such as Lineweaver–Burk), identification of the inhibition type (competitive, noncompetitive, or uncompetitive), and calculation of Ki from observed parameter changes. Expect students to state assumptions (steady-state, initial rates), show algebraic steps or fitting rationale, and comment on fit quality and potential experimental error.