Assess students' understanding of how farmland, neighborhoods, and factories affect a local stream's water quality and aquatic wildlife. Items cover common pollution sources (nutrients, pesticides, sediment, urban runoff, industrial discharge), effects on pH, dissolved oxygen, turbidity, and food webs, use of bioindicator species, and basic mitigation practices (riparian buffers, stormwater management, treatment). Includes scenario-based questions requiring cause–effect explanations and interpretation of simple water-quality data.