Assess students' ability to analyze steady-state and pre-steady-state kinetic data to distinguish Michaelis–Menten, allosteric, and cooperative mechanisms; tasks include estimating kcat and Km, applying diagnostic plots and fits (Michaelis–Menten, Hill), interpreting Hill coefficients and sigmoidal vs hyperbolic velocity curves, identifying burst phases or transients from rapid‑mixing data, and proposing experimental tests/controls to discriminate conformational regulation from multisite cooperativity.