Assess the learner's ability to design, execute, and interpret enzyme inhibition assays using Michaelis-Menten kinetics. Expect tasks to include selecting appropriate substrate and inhibitor concentration ranges, measuring initial velocities, distinguishing competitive, noncompetitive, and uncompetitive inhibition by analyzing changes in Km and Vmax, fitting data with Lineweaver-Burk and nonlinear regression to estimate Km, Vmax, and Ki, and identifying necessary controls and common experimental pitfalls. Provide sample velocity data for parameter calculation and require a reasoned conclusion about inhibitor mechanism and assay design choices.