Assess undergraduate understanding of Michaelis–Menten kinetics and enzyme regulation: test derivation assumptions, interpretation and calculation of KM, Vmax, kcat and catalytic efficiency (kcat/KM), reading kinetic plots (Lineweaver–Burk, Eadie–Hofstee), and predicting how competitive, noncompetitive, uncompetitive and mixed inhibitors change parameters; include mechanistic questions on allosteric modulation, covalent modification, feedback inhibition and zymogen activation, plus data-driven problems requiring parameter estimation and interpretation.