Assess graduate-level understanding of Michaelis-Menten enzyme kinetics and Lineweaver-Burk linearization by requiring learners to derive and interpret Km and Vmax from velocity-versus-substrate data, explain and evaluate core assumptions (steady-state, single-substrate, initial-rate conditions), identify common limitations and pitfalls (error amplification in double-reciprocal plots, non-Michaelian behavior, inhibition types, cooperativity, reversible or multi-step mechanisms), and justify appropriate analysis choices (nonlinear regression versus linear transforms) for accurate parameter estimation and interpretation.