Assess students' ability to determine Km and Vmax from initial velocity versus substrate concentration data using Michaelis–Menten analysis; tasks include selecting an appropriate fitting method (nonlinear regression and at least one linear transformation such as Lineweaver–Burk or Eadie–Hofstee), constructing plots, calculating Km and Vmax with correct units, estimating catalytic efficiency where kcat is available, and interpreting deviations or experimental error that affect parameter estimates. Provide clear calculation steps, show fits and residuals, and justify choice of analysis.