Assess ability to apply Michaelis–Menten kinetics and classical inhibition models to predict and calculate reaction velocities in multi‑substrate enzymatic systems; includes deriving and using steady‑state rate equations for ordered, random and ping‑pong mechanisms, distinguishing competitive, uncompetitive, noncompetitive and mixed inhibition in multi‑substrate contexts, calculating v from Km, Vmax, Ki and substrate/inhibitor concentrations, and interpreting Lineweaver–Burk and secondary plots for parameter estimation.