Assess students' ability to analyze experimental initial-rate data for an enzyme with and without inhibitors to identify inhibition type (competitive, noncompetitive, uncompetitive), calculate Km and Vmax and inhibition constants (Ki) using appropriate linear transformations and/or nonlinear regression (Michaelis–Menten, Lineweaver–Burk, Eadie–Hofstee), justify the chosen model and show calculations, and discuss assumptions and sources of error.