Assess students' ability to analyze initial-rate enzyme kinetics measured at multiple substrate and inhibitor concentrations to (a) identify inhibitor mechanism (competitive, uncompetitive, noncompetitive/mixed) using appropriate plots or nonlinear regression, (b) calculate inhibition constants (Ki and Ki' when relevant) with clear derivations or fitting procedures, and (c) interpret changes in Km and Vmax and note experimental considerations (tight-binding, substrate depletion, assay design) that affect conclusions.