Assess undergraduate students' understanding of enzyme-catalyzed reaction kinetics and mechanisms, including interpretation and calculation of Michaelis–Menten parameters (Km, Vmax, kcat), use of linear transformations (e.g., Lineweaver–Burk), identification of competitive, noncompetitive, uncompetitive and mixed inhibition from kinetic data, and how pH, temperature, substrate concentration, cofactors, and allosteric regulation affect catalytic efficiency (kcat/Km). Problems may combine data analysis, calculations, and conceptual explanation.