Assess ability to analyze initial-rate enzymatic data collected at multiple substrate and inhibitor concentrations to identify inhibition mechanism (competitive, noncompetitive, uncompetitive, mixed). Students will apply Michaelis–Menten equations and linear transformations (Lineweaver–Burk, Eadie–Hofstee) and nonlinear curve-fitting to estimate Km, Vmax, Ki, interpret parameter changes and plots, and justify mechanism assignments while noting experimental limitations.