Assess students' ability to analyze enzyme-catalyzed initial-rate vs substrate-concentration data using Michaelis–Menten kinetics: calculate initial rates, choose and apply appropriate fitting methods (nonlinear regression and common linearizations such as Lineweaver–Burk or Eadie–Hofstee), estimate Vmax and Km with units and uncertainty, evaluate fit quality and underlying assumptions (steady-state, initial-rate conditions, single-substrate behavior), and interpret biological implications and limitations of the estimates.