Assess students' ability to analyze enzyme assay initial-rate data to estimate Km and Vmax and determine inhibition constants (Ki) using nonlinear regression; tasks include choosing and fitting appropriate models (Michaelis–Menten; competitive, noncompetitive, uncompetitive, mixed inhibition), applying proper error weighting, comparing fits using residuals and information criteria, reporting parameter estimates with confidence intervals, and justifying the inferred inhibition mechanism based on kinetic behavior and diagnostic plots.